Chemical Oxidation of HPA
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When we were unsuccessful with the oxidation of oxybenzone both enzymatically and chemically, we turned to HPA for chemical oxidation.
Making the Samples For Chemical Oxidation
2 mM HPA Buffer Stock Solution
Dissolved 30 mg of HPA in 100 mL of ph 5 buffer solution.
Potassium Permanganate Stock Solution
Preparing the final samples
Measured out 5 mL of the 2 mM HPA stock solution into 4 different scintillation vials. Added potassium permanganate solution in increasing concentrations to each vial (6 microliters, 12 microliters, 18 microliters, and 24 microliters).