Chemical Oxidation of HPA

When we were unsuccessful with the oxidation of oxybenzone both enzymatically and chemically, we turned to HPA for chemical oxidation.

Making the Samples For Chemical Oxidation

2 mM HPA Buffer Stock Solution

Dissolved 30 mg of HPA in 100 mL of ph 7 buffer solution.

Potassium Permanganate Stock Solution

2mM Potassium Permanganate Stock Solution

• Dissolved 0.0316 g of potassium permanganate in 100 mL of RO water.

4mM Potassium Permanganate Stock Solution

• Dissolved 0.0632 g of potassium permanganate in 100 mL of RO water.

10mM Potassium Permanganate Stock Solution

• Dissolved 0.158 g of potassium permanganate in 100 mL of RO water.

20mM Potassium Permanganate Stock Solution

• Dissolved 0.316 g of potassium permanganate in 100 mL of RO water.

Preparing the final samples

Added 5 mL of the oxybenzone buffer stock solution to a scintillation vial. Then added 5 mL of the 1 mM Potassium Permanganate Stock Solution to the same vial that contained the oxybenzone buffer solution. Mixed solution thoroughly. This vial now contained 1 mM HPA solution and 1 mM potassium permanganate solution once dilution was accounted for. This process was repeated with the rest of the potassium permanganate solutions.

g KMnO4	Concentration of Stock Solution KMnO4 (mM)	Concentration of KMnO4 After Dilution (mM)	Concentration of HPA After Dilution (mM)
0.0316	2	1	1
0.0632	4	2	1
0.158	10	5	1
0.316	20	10	1