Synthesis and Characterization of Lignin Monomers

Prior research on Synthesis of Lignin Monomers done by: Alexandria N. Tibbs

Abstract

Lignin is the second most abundant organic polymer. The structure of lignin is composed of three monomers: p-coumaryl alcohol, coniferyl alcohol, and sinapyl alcohol, which all are found in nature. These natural compounds are not cost friendly for undergraduate research. The carboxylic acid forms of these monomers (p-coumaric acid, ferulic acid, and sinapic acid) can be obtained commercially. This poster will discuss our synthetic approaches to produce the alcohol forms of lignin.

Lignin Monomers

p-Coumaryl alcohol

Lignin Precursors

Coniferyl alcohol

Sinapyl alcohol

Introduction

Ferulates play important roles in plant growth and undergo radical coupling reactions to produce dimers and cross-linking. Significant quantities are now required by cell wall researchers increasing the the large scale production of these ferulates and other lignins similar to their make up.

Methods/Experimentation

Materials

Ferulic acid

p-Coumaric acid

Sinapic acid

Experimental Procedures

Preparation through 8--5 Coupled Diferulate Procedure ()

• Ethyl Ferulate: 10 g of ferulic acid dissolved in 100 mL ethanol and 5 mL of acetyl chloride. Solution stirred overnight. Volatiles removed by rotary evaporation (rotovap) at 40 C.
• Dimerization: Product of ethyl ferulate dissolved in pH 4.0 acetate buffer. Solution is cooled and added to peroxidase(10 mg in 2 mL of phosphate buffer). Product filtered out to obtain crude product.
• Purification: Use of flash chromatography with EtOAc as eluant. Product recrystallized allowing for NMR data and melting point.

• Esterification: 0.36 M ferulic acid in ethanol (500 mL) with concentrated HCl. Refluxed for two days. Cooled and ethanol evaporated under pressure (rotovap). Crude extract combined with ethyl acetate (250 mL). Solution dried over anhydrous MgSO₄.

Results

While minimal research was able to be accomplished, discussion on methods were able to be looked at to finding a more green chemistry approach. The initial method allowed for a two day reflux accumulating in a significant amount of water being used (roughly 700+ gallons).

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Conclusion

References

1. [Overview from Lignin and Lignans: Advances in Chemistry]

2. Lignin Biosynthesis and Structure

3. Oxidative Coupling during Lignin Polymerization is Determined by Unpaired Electron Delocalization within Parent Phenylpropanoid Radicals

4. Facile Large-Scale Synthesis of Coniferyl, Sinapyl, and p-Coumaryl Alcohol

5. Simple Preparation of 8--5-coupled Diferulate

• PDF Version: File:Simple Preparation of 8-5 Coupled Diferu.pdf

6. Structure-Activity Relationships

• PDF Version: File:Structure-Activity Relationships.pdf