Difference between revisions of "Chlorogenic acid isolation"
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9/22 | 9/22 | ||
| − | Ground green coffee beans with a blade grinder. Gather the 35 mesh and 60 mesh sized particles. Made 500 mL of 70/30 methanol/water. Shook two 50 mL tubes of .5 g of the green coffee bean particles and 50 mL methanol/water for 1 hour. After shaking let the green coffee bean particles settle, then ran a TLC plate with a CGA standard on it. Method works! | + | Ground green coffee beans with a blade grinder. Gather the 35 mesh and 60 mesh sized particles. Made 500 mL of 70/30 methanol/water. Shook two 50 mL tubes of .5 g of the green coffee bean particles and 50 mL methanol/water for 1 hour. After shaking let the green coffee bean particles settle, then ran a [[CGA_TLC|TLC]] plate with a CGA standard on it. Method works! |
9/27 | 9/27 | ||
| − | + | Shook 1 tube of the 35 mesh for two hours to see if time shaken effects the amount of CGA extracted. 1 more [[CGA_TLC|TLC]] was performed with thee two 35 mesh samples. | |
Revision as of 18:43, 27 September 2016
Introduction
Chlorogenic acid (CGA) has been said to have beneficial biological properties such as lowering blood pressure and a laxative effect. CGA can be found in several plant like prunes, eggplants, peaches, bamboo and green coffee beans. CGA has been trademarked as Svetol, and is added to many food products like chewing gum and mints.
Chlorogenic acids are being used in health drinks and in high demand, finding a better way to extract the CGA would be big
Proposed Project Description
Extraction chlorogenic acid from green coffee beans. Purification of the crude extract with flash chromatography, and refining the process improving upon it.
References
1):Five ways to Isolate Chlorogenic acid.
2):Microwave assisted extraction with other references to standard solvent extractions.
Current Progress
Sept 2016
9/20 Extraction method
9/22 Ground green coffee beans with a blade grinder. Gather the 35 mesh and 60 mesh sized particles. Made 500 mL of 70/30 methanol/water. Shook two 50 mL tubes of .5 g of the green coffee bean particles and 50 mL methanol/water for 1 hour. After shaking let the green coffee bean particles settle, then ran a TLC plate with a CGA standard on it. Method works!
9/27 Shook 1 tube of the 35 mesh for two hours to see if time shaken effects the amount of CGA extracted. 1 more TLC was performed with thee two 35 mesh samples.