Chlorogenic acid isolation
Introduction
Chlorogenic acid (CGA) has been said to have beneficial biological properties such as lowering blood pressure and a laxative effect. CGA can be found in several plant like prunes, eggplants, peaches, bamboo and green coffee beans. CGA has been trademarked as Svetol, and is added to many food products like chewing gum and mints.
Chlorogenic acids are being used in health drinks and in high demand, finding a better way to extract the CGA would be big
Proposed Project Description
Extraction chlorogenic acid from green coffee beans. Purification of the crude extract with flash chromatography, and refining the process improving upon it.
References
1):Five ways to Isolate Chlorogenic acid.
2):Microwave assisted extraction with other references to standard solvent extractions.
Current Progress
Sept 2016
9/20 Extraction method
9/22 Ground green coffee beans with a blade grinder. Gather the 35 mesh and 60 mesh sized particles. Made 500 mL of 70/30 methanol/water. Shook two 50 mL tubes of .5 g of the green coffee bean particles and 50 mL methanol/water for 1 hour. After shaking let the green coffee bean particles settle, then ran a TLC plate with a CGA standard on it. Method works!
9/27 Shook 1 tube of the 35 mesh for two hours to see if time shaken effects the amount of CGA extracted. 1 more TLC was performed with thee two 35 mesh samples.
9/28 First use the UV-Vis, to find a spectra of the sample, finding a maximum at about 330. Then ran a flash column of with a silica column with ethyl acetate and hexanes as the mobile phase nothing flowed off of the column after thirty minutes of run time. After flushing the column with water it flow off of the column. The ran a TLC with the sample to compare the eluding power of ethyl acetate to methanol.
9/29 Ran another flash column with a C18 column, with acidified water and acetonitrile as mobile phases. The first run had sample flow out in the very beginning of the run at 100% water, the second run started at 50% water 50% acetonitrile and again it ran off very early, the third and final attempt started with 100% acetonitrile and still it flowed off the column in the very beginning of the run. While running the samples the sample was filtered to leave the green bean particles behind and then 15ml was placed in a vial in a freezer to be lyophilized.
Feb 2017
2/1 Purification method - After the crude is extracted it is roto-vaped to evaporate the methanol, and washed twice with chloroform to pull out the caffeine and wax and washed again with Pet-Ether two more times to pull out the lipids and pigments
2/7 HPLC data was collected and examined
2/13 NMR Trials are being done for the rest of this week