Difference between revisions of "Oxidation of Acetaminophen"
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|+Summary of Hyperfine Coupling | |+Summary of Hyperfine Coupling | ||
|-bgcolor=gray | |-bgcolor=gray | ||
| − | !Nucle (# of spins) | + | !Nucle (# of spins)!!hyperfine!!hyperfine!!hyperfine |
|- | |- | ||
| − | |Ref --> ||West, 1984||Fischer, 1986||Sturgeon, not published | + | |Ref --> ||align=center|West, 1984||align=center|Fischer, 1986||align=center|Sturgeon, not published |
| + | |- | ||
| + | |Conditions ||align=center|fast-flow, pH 7.5||align=center|fast-flow, pH 7.5||align=center|immoblized-Enzyme, pH 5/dioxane (50/50) | ||
|- | |- | ||
|Spectrum ||unresolved triplet||high res multiline||med res multiline | |Spectrum ||unresolved triplet||high res multiline||med res multiline | ||
|- | |- | ||
| − | | N (1) || nr|| 0.81 G ||1.68 G | + | | N (1) || align=center|nr|| align=center|0.81 G ||align=center|1.68 G |
|- | |- | ||
| − | | H, on nitrogen (1) || nr || 1.35 G ||0.96 G | + | | H, on nitrogen (1) || align=center|nr || align=center|1.35 G ||align=center|0.96 G |
|- | |- | ||
| − | | H, ortho (2) || 5.2 G|| 5.11 G || 5.19 G | + | | H, ortho (2) || align=center|5.2 G|| align=center|5.11 G || align=center|5.19 G |
|- | |- | ||
| − | | H, meta (2) || nr || 0.64 G|| 0.76 G | + | | H, meta (2) ||align=center| nr || align=center|0.64 G|| align=center|0.76 G |
|- | |- | ||
| − | | H, | + | | H, methyl (3) ||align=center|nr || align=center|1.01 G ||align=center|nr |
|} | |} | ||
nr = not reported or not resolved | nr = not reported or not resolved | ||
| − | |||
| − | |||
| − | |||
==HRP/H2O2== | ==HRP/H2O2== | ||
| Line 30: | Line 29: | ||
|[[File:APAP rad HRP.png|200px|thumb|center|West 1984, Fischer,1985]] | |[[File:APAP rad HRP.png|200px|thumb|center|West 1984, Fischer,1985]] | ||
||[[File:Screen Shot 2018-07-15 at 7.38.54 AM.png|230px|thumb|center|Fischer 1986]] | ||[[File:Screen Shot 2018-07-15 at 7.38.54 AM.png|230px|thumb|center|Fischer 1986]] | ||
| − | ||[[File:WIU FigX.jpg| | + | ||[[File:WIU FigX.jpg|230px|thumb|center|Sturgeon, unpublished]] |
|} | |} | ||
Latest revision as of 01:00, 16 July 2018
Summary of ESR Data
| Nucle (# of spins) | hyperfine | hyperfine | hyperfine |
|---|---|---|---|
| Ref --> | West, 1984 | Fischer, 1986 | Sturgeon, not published |
| Conditions | fast-flow, pH 7.5 | fast-flow, pH 7.5 | immoblized-Enzyme, pH 5/dioxane (50/50) |
| Spectrum | unresolved triplet | high res multiline | med res multiline |
| N (1) | nr | 0.81 G | 1.68 G |
| H, on nitrogen (1) | nr | 1.35 G | 0.96 G |
| H, ortho (2) | 5.2 G | 5.11 G | 5.19 G |
| H, meta (2) | nr | 0.64 G | 0.76 G |
| H, methyl (3) | nr | 1.01 G | nr |
nr = not reported or not resolved
HRP/H2O2
- ACETAMINOPHEN - ENZYMATIC FORMATION OF A TRANSIENT PHENOXYL FREE-RADICAL
- West, PR, Harman, LS, Josephy, PD, Mason, RP
- Biochemical pharmacology, 1984, Vol.33(18), pp.2933-2936.
- Free-Radical Metabolites of Acetaminophen and a Dimethylated Derivative
- Volker Fischer, Paul R. West, Laura S. Harman, and Ronald P. Mason.
- Environmental Health Perspectives, 1985, 64, pp. 127-137.
- Direct electron spin resonance detection of free radical intermediates during the peroxidase catalyzed oxidation of phenacetin metabolites.
- Volker Fischer, Laura S. Harman, Paul R. West, and Ronald P. Mason.
- Chem.-Biol. Interactions, 1986, Vol 60, PP. 115-127.
HRP/H2O2/Acetaminophen analog
- Stable Free Radical and Benzoquinone Imine Metabolites of an Acetaminophen Analogue
- Volker Fischer and Ronald P. Mason.
- Journal of Biological Chemistry, 1984, 259(16), pp. 10284-10288.
- here
with GSH
Oxidation Product Analysis
- Identification of Acetaminophen Polymerization Products Catalyzed by Horseradish Peroxidase.
- David W. Potter$, Dwight W. Miller, and Jack A. Hinson
- Journal of Biological Chemistry, 1985 "260(22), pp 12174-12180.
- here
