Summary of ESR Data
Summary of Hyperfine Coupling
| Nucle (# of spins) |
hyperfine |
hyperfine |
hyperfine
|
| Ref --> |
West, 1984 |
Fischer, 1986 |
Sturgeon, not published
|
| Conditions |
fast-flow, pH 7.5 |
fast-flow, pH 7.5 |
immoblized-Enzyme, pH 5/dioxane (50/50)
|
| Spectrum |
unresolved triplet |
high res multiline |
med res multiline
|
| N (1) |
nr |
0.81 G |
1.68 G
|
| H, on nitrogen (1) |
nr |
1.35 G |
0.96 G
|
| H, ortho (2) |
5.2 G |
5.11 G |
5.19 G
|
| H, meta (2) |
nr |
0.64 G |
0.76 G
|
| H, methyl (3) |
nr |
1.01 G |
nr
|
nr = not reported or not resolved
HRP/H2O2
- ACETAMINOPHEN - ENZYMATIC FORMATION OF A TRANSIENT PHENOXYL FREE-RADICAL
- West, PR, Harman, LS, Josephy, PD, Mason, RP
- Biochemical pharmacology, 1984, Vol.33(18), pp.2933-2936.
- Free-Radical Metabolites of Acetaminophen and a Dimethylated Derivative
- Volker Fischer, Paul R. West, Laura S. Harman, and Ronald P. Mason.
- Environmental Health Perspectives, 1985, 64, pp. 127-137.
- Direct electron spin resonance detection of free radical intermediates during the peroxidase catalyzed oxidation of phenacetin metabolites.
- Volker Fischer, Laura S. Harman, Paul R. West, and Ronald P. Mason.
- Chem.-Biol. Interactions, 1986, Vol 60, PP. 115-127.
HRP/H2O2/Acetaminophen analog
- Stable Free Radical and Benzoquinone Imine Metabolites of an Acetaminophen Analogue
- Volker Fischer and Ronald P. Mason.
- Journal of Biological Chemistry, 1984, 259(16), pp. 10284-10288.
- here
with GSH
- Media:J. Biol. Chem.-1990-Ramakrishna Rao-844-7.pdf
Oxidation Product Analysis
- Identification of Acetaminophen Polymerization Products Catalyzed by Horseradish Peroxidase.
- David W. Potter$, Dwight W. Miller, and Jack A. Hinson
- Journal of Biological Chemistry, 1985 "260(22), pp 12174-12180.
- here
Related Phenacetin
http://www.ncbi.nlm.nih.gov/pubmed/3024853
