Difference between revisions of "Chemical Oxidation of HPA"

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20mM Potassium Permanganate Stock Solution
 
20mM Potassium Permanganate Stock Solution
 
* Dissolved 0.316 g of potassium permanganate in 100 mL of RO water.
 
* Dissolved 0.316 g of potassium permanganate in 100 mL of RO water.
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==== Preparing the final samples ====
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Added 5 mL of the oxybenzone buffer stock solution to a scintillation vial. Then added 5 mL of the 1 mM Potassium Permanganate Stock Solution to the same vial that contained the oxybenzone buffer solution. Mixed solution thoroughly. This vial now contained 1 mM HPA solution and 1 mM potassium permanganate solution once dilution was accounted for. This process was repeated with the rest of the potassium permanganate solutions.
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{| class="wikitable"
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|-
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! g KMnO4 !! Concentration of Stock Solution KMnO4 (mM) !! Concentration of KMnO4 After Dilution (mM) !! Concentration of HPA After Dilution (mM)
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|-
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| 0.0316 || 2 || 1 || 1
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|-
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| 0.0632 || 4 || 2 || 1
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|-
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| 0.158|| 10 || 5 || 1
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|-
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| 0.316 || 20 || 10 || 1
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|}

Latest revision as of 21:13, 30 June 2020

When we were unsuccessful with the oxidation of oxybenzone both enzymatically and chemically, we turned to HPA for chemical oxidation.

Making the Samples For Chemical Oxidation

2 mM HPA Buffer Stock Solution

Dissolved 30 mg of HPA in 100 mL of ph 7 buffer solution.

Potassium Permanganate Stock Solution

2mM Potassium Permanganate Stock Solution

  • Dissolved 0.0316 g of potassium permanganate in 100 mL of RO water.

4mM Potassium Permanganate Stock Solution

  • Dissolved 0.0632 g of potassium permanganate in 100 mL of RO water.

10mM Potassium Permanganate Stock Solution

  • Dissolved 0.158 g of potassium permanganate in 100 mL of RO water.

20mM Potassium Permanganate Stock Solution

  • Dissolved 0.316 g of potassium permanganate in 100 mL of RO water.

Preparing the final samples

Added 5 mL of the oxybenzone buffer stock solution to a scintillation vial. Then added 5 mL of the 1 mM Potassium Permanganate Stock Solution to the same vial that contained the oxybenzone buffer solution. Mixed solution thoroughly. This vial now contained 1 mM HPA solution and 1 mM potassium permanganate solution once dilution was accounted for. This process was repeated with the rest of the potassium permanganate solutions.


g KMnO4 Concentration of Stock Solution KMnO4 (mM) Concentration of KMnO4 After Dilution (mM) Concentration of HPA After Dilution (mM)
0.0316 2 1 1
0.0632 4 2 1
0.158 10 5 1
0.316 20 10 1