Difference between revisions of "Chemical Oxidation of HPA"
| (2 intermediate revisions by the same user not shown) | |||
| Line 8: | Line 8: | ||
''Potassium Permanganate Stock Solution'' | ''Potassium Permanganate Stock Solution'' | ||
| − | |||
| − | |||
2mM Potassium Permanganate Stock Solution | 2mM Potassium Permanganate Stock Solution | ||
| − | * | + | * Dissolved 0.0316 g of potassium permanganate in 100 mL of RO water. |
| − | + | 4mM Potassium Permanganate Stock Solution | |
| − | * | + | * Dissolved 0.0632 g of potassium permanganate in 100 mL of RO water. |
10mM Potassium Permanganate Stock Solution | 10mM Potassium Permanganate Stock Solution | ||
| − | * | + | * Dissolved 0.158 g of potassium permanganate in 100 mL of RO water. |
| − | + | 20mM Potassium Permanganate Stock Solution | |
| + | * Dissolved 0.316 g of potassium permanganate in 100 mL of RO water. | ||
| + | |||
| + | ==== Preparing the final samples ==== | ||
| + | |||
| + | Added 5 mL of the oxybenzone buffer stock solution to a scintillation vial. Then added 5 mL of the 1 mM Potassium Permanganate Stock Solution to the same vial that contained the oxybenzone buffer solution. Mixed solution thoroughly. This vial now contained 1 mM HPA solution and 1 mM potassium permanganate solution once dilution was accounted for. This process was repeated with the rest of the potassium permanganate solutions. | ||
| + | |||
| + | |||
| + | {| class="wikitable" | ||
| + | |- | ||
| + | ! g KMnO4 !! Concentration of Stock Solution KMnO4 (mM) !! Concentration of KMnO4 After Dilution (mM) !! Concentration of HPA After Dilution (mM) | ||
| + | |- | ||
| + | | 0.0316 || 2 || 1 || 1 | ||
| + | |- | ||
| + | | 0.0632 || 4 || 2 || 1 | ||
| + | |- | ||
| + | | 0.158|| 10 || 5 || 1 | ||
| + | |- | ||
| + | | 0.316 || 20 || 10 || 1 | ||
| + | |} | ||
Latest revision as of 21:13, 30 June 2020
When we were unsuccessful with the oxidation of oxybenzone both enzymatically and chemically, we turned to HPA for chemical oxidation.
Making the Samples For Chemical Oxidation
2 mM HPA Buffer Stock Solution
Dissolved 30 mg of HPA in 100 mL of ph 7 buffer solution.
Potassium Permanganate Stock Solution
2mM Potassium Permanganate Stock Solution
- Dissolved 0.0316 g of potassium permanganate in 100 mL of RO water.
4mM Potassium Permanganate Stock Solution
- Dissolved 0.0632 g of potassium permanganate in 100 mL of RO water.
10mM Potassium Permanganate Stock Solution
- Dissolved 0.158 g of potassium permanganate in 100 mL of RO water.
20mM Potassium Permanganate Stock Solution
- Dissolved 0.316 g of potassium permanganate in 100 mL of RO water.
Preparing the final samples
Added 5 mL of the oxybenzone buffer stock solution to a scintillation vial. Then added 5 mL of the 1 mM Potassium Permanganate Stock Solution to the same vial that contained the oxybenzone buffer solution. Mixed solution thoroughly. This vial now contained 1 mM HPA solution and 1 mM potassium permanganate solution once dilution was accounted for. This process was repeated with the rest of the potassium permanganate solutions.
| g KMnO4 | Concentration of Stock Solution KMnO4 (mM) | Concentration of KMnO4 After Dilution (mM) | Concentration of HPA After Dilution (mM) |
|---|---|---|---|
| 0.0316 | 2 | 1 | 1 |
| 0.0632 | 4 | 2 | 1 |
| 0.158 | 10 | 5 | 1 |
| 0.316 | 20 | 10 | 1 |