Difference between revisions of "Chemical Oxidation of HPA"
(Created page with "When we were unsuccessful with the oxidation of oxybenzone both enzymatically and chemically, we turned to HPA for chemical oxidation. ==Making the Samples For Chemical Oxid...") |
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''2 mM HPA Buffer Stock Solution'' | ''2 mM HPA Buffer Stock Solution'' | ||
| − | Dissolved 30 mg of HPA in 100 mL of ph | + | Dissolved 30 mg of HPA in 100 mL of ph 7 buffer solution. |
''Potassium Permanganate Stock Solution'' | ''Potassium Permanganate Stock Solution'' | ||
| + | 2mM Potassium Permanganate Stock Solution | ||
| + | * Dissolved 0.0316 g of potassium permanganate in 100 mL of RO water. | ||
| − | + | 4mM Potassium Permanganate Stock Solution | |
| + | * Dissolved 0.0632 g of potassium permanganate in 100 mL of RO water. | ||
| − | + | 10mM Potassium Permanganate Stock Solution | |
| + | * Dissolved 0.158 g of potassium permanganate in 100 mL of RO water. | ||
| + | |||
| + | 20mM Potassium Permanganate Stock Solution | ||
| + | * Dissolved 0.316 g of potassium permanganate in 100 mL of RO water. | ||
| + | |||
| + | ==== Preparing the final samples ==== | ||
| + | |||
| + | Added 5 mL of the oxybenzone buffer stock solution to a scintillation vial. Then added 5 mL of the 1 mM Potassium Permanganate Stock Solution to the same vial that contained the oxybenzone buffer solution. Mixed solution thoroughly. This vial now contained 1 mM HPA solution and 1 mM potassium permanganate solution once dilution was accounted for. This process was repeated with the rest of the potassium permanganate solutions. | ||
| + | |||
| + | |||
| + | {| class="wikitable" | ||
| + | |- | ||
| + | ! g KMnO4 !! Concentration of Stock Solution KMnO4 (mM) !! Concentration of KMnO4 After Dilution (mM) !! Concentration of HPA After Dilution (mM) | ||
| + | |- | ||
| + | | 0.0316 || 2 || 1 || 1 | ||
| + | |- | ||
| + | | 0.0632 || 4 || 2 || 1 | ||
| + | |- | ||
| + | | 0.158|| 10 || 5 || 1 | ||
| + | |- | ||
| + | | 0.316 || 20 || 10 || 1 | ||
| + | |} | ||
Latest revision as of 21:13, 30 June 2020
When we were unsuccessful with the oxidation of oxybenzone both enzymatically and chemically, we turned to HPA for chemical oxidation.
Making the Samples For Chemical Oxidation
2 mM HPA Buffer Stock Solution
Dissolved 30 mg of HPA in 100 mL of ph 7 buffer solution.
Potassium Permanganate Stock Solution
2mM Potassium Permanganate Stock Solution
- Dissolved 0.0316 g of potassium permanganate in 100 mL of RO water.
4mM Potassium Permanganate Stock Solution
- Dissolved 0.0632 g of potassium permanganate in 100 mL of RO water.
10mM Potassium Permanganate Stock Solution
- Dissolved 0.158 g of potassium permanganate in 100 mL of RO water.
20mM Potassium Permanganate Stock Solution
- Dissolved 0.316 g of potassium permanganate in 100 mL of RO water.
Preparing the final samples
Added 5 mL of the oxybenzone buffer stock solution to a scintillation vial. Then added 5 mL of the 1 mM Potassium Permanganate Stock Solution to the same vial that contained the oxybenzone buffer solution. Mixed solution thoroughly. This vial now contained 1 mM HPA solution and 1 mM potassium permanganate solution once dilution was accounted for. This process was repeated with the rest of the potassium permanganate solutions.
| g KMnO4 | Concentration of Stock Solution KMnO4 (mM) | Concentration of KMnO4 After Dilution (mM) | Concentration of HPA After Dilution (mM) |
|---|---|---|---|
| 0.0316 | 2 | 1 | 1 |
| 0.0632 | 4 | 2 | 1 |
| 0.158 | 10 | 5 | 1 |
| 0.316 | 20 | 10 | 1 |