Difference between revisions of "Chemical Oxidation of HPA"

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(Created page with "When we were unsuccessful with the oxidation of oxybenzone both enzymatically and chemically, we turned to HPA for chemical oxidation. ==Making the Samples For Chemical Oxid...")
 
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''2 mM HPA Buffer Stock Solution''
 
''2 mM HPA Buffer Stock Solution''
  
Dissolved 30 mg of HPA in 100 mL of ph 5 buffer solution.
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Dissolved 30 mg of HPA in 100 mL of ph 7 buffer solution.
  
 
''Potassium Permanganate Stock Solution''
 
''Potassium Permanganate Stock Solution''
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1mM Potassium Permanganate Stock Solution
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2mM Potassium Permanganate Stock Solution
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* Bulleted list item
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5mM Potassium Permanganate Stock Solution
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10mM Potassium Permanganate Stock Solution
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* Bulleted list item
  
 
''Preparing the final samples''
 
''Preparing the final samples''
 
Measured out 5 mL of the 2 mM HPA stock solution into 4 different scintillation vials. Added potassium permanganate solution in increasing concentrations to each vial (6 microliters, 12 microliters, 18 microliters, and 24 microliters).
 

Revision as of 20:53, 13 February 2020

When we were unsuccessful with the oxidation of oxybenzone both enzymatically and chemically, we turned to HPA for chemical oxidation.

Making the Samples For Chemical Oxidation

2 mM HPA Buffer Stock Solution

Dissolved 30 mg of HPA in 100 mL of ph 7 buffer solution.

Potassium Permanganate Stock Solution 1mM Potassium Permanganate Stock Solution

  • Bulleted list item

2mM Potassium Permanganate Stock Solution

  • Bulleted list item

5mM Potassium Permanganate Stock Solution

  • Bulleted list item

10mM Potassium Permanganate Stock Solution

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Preparing the final samples