Tissue Printing

Tissue printing can be used to visualize Peroxidase in vegetables, fruits, and mushrooms as well as other enzymes and protein by changing the membrane in which they bind to.

Tissue printing for Peroxidase

The membrane used for peroxidase printing is a Nitrocellulose membrane. This membrane adsorbs large quantities of proteins that are tightly bound, but it does not retain salts and small hydrophobic molecules very well. Hot-press watercolor paper has also been used for this procedure as it has a very smooth surface and holds on to moisture of the organism. By pressing the vegetable or fruit with slight pressure onto the nitrocellulose of paper surface, one can leave a residue of liquid and proprieties. To visualize the peroxidase, the paper is sprayed with a ABTS, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), buffer solution. In the presence of peroxidase, a blue-green color with become present. The are in Which the reaction occurs can be compartmentalized on the print, and specific shapes can be seen with a magnified glass or a microscope. In plant systems, peroxidase is likely to play a role in synthesis of the plant cell wall.

Question: what is the concentration of ABTS?