Bradley Dulee Chem430 F16

Chemistry/Biochemistry Research 430

Fall 2016

Brad Dulee

Senior Biochemistry Major

Research Times

TBD - 4 hours per week

section 01 = 0.25 credit = 4 hours per week.

Proposed Research Project

General Information

Advisor: Laura Moore

Other research student collaborators: Tyler Bailey

Proposal

It is the goal of this project to focus on increasing the tolerance of Escherichia coli against the harmful byproducts of lignocellulosic biomass pretreatment methods for use in biofuel production.

Instruments to be used

- I2400 incubator shaker (37°C)

- Allegra 25R Centrifuge (4°C)

- Bio Rad MicroPulser Electroporator

- Multiwell FC Microwell Plate Reader

- Nanodrop

References (2 minimum)

1. Mills, T. Y.; Sandoval, N. R.; Gill, R. T. Cellulosic hydrolysate toxicity and tolerance mechanisms in "Escherichia coli". "Biotechnology for Biofuels" 2009, 2:26.

2. Wang, X.; Miller, E. N.; Yomano, L. P.; Zhang, X.; Shanmugam, K. T.; Ingram, L. O. Increased Furfural Tolerance Due to Overexpression of NADH-Dependent Oxidoreductase FucO in "Escherichia coli" Strains Engineered for the Production of Ethanol and Lactate. "Applied and Environment Biology" 2011, 77:15, 5132-5140.

3. Richardson,T. L.; Harner, N. K.; Bajwa, P. K.; Trevors, J. T.; Lee, H. Approaches to Deal with Toxic Inhibitors During Fermentation of Lignocellulosic Substrates. "ACS Symposium Series". 2011, 1067, Chapter 7, 171-202.

4. Olsson, L.; Hahn-Hägerdal, B.; Fermentation of Lignocellulosic Hydrosylates for Ethanol Production. "Enzyme and Microbial Technology". 1996, 18, 312-331.

5. Wang, X.; Miller, E. N.; Yomano, L. P.; Zhang, X.; Shanmugam, K. T.; Ingram, L. O. Increased Furan Tolerance in "Escherichia Coli" Due to a Cryptic ucpA Gene. "Applied and Environmental Microbiology". 2012, 78:7, 2452-2455.

6. Weil, J. R.; Dien, B.; Bothast, R.; Hendrickson, R.; Mosier, N. S.; Ladisch, M. R. Removal of Fermentation Inhibitors Formed during Pretreatment of Biomass by Polymeric Adsorbents. "Ind. Eng. Chem." Res. 2002, 41:24, 6132-6138.

Research pledge

I, Brad Dulee, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.