Caffeine HPLC Method

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HPLC method for caffeine analysis

High Pressure Liquid Chromatography Setup (HPLC)

HPLC Pump Gradient Flow Settings
HPLC Pump Solvent Settings
HPLC UV Detector General Settings
HPLC UV Detector Channel 1 Settings
HPLC UV Detector Channel 2 Settings

Results

Caffeine should be detected as sharp peaks which elute at approximately 11 minutes when monitoring 275nm on the UV Detector.

The image below is the spectra of a caffeine standard run with the above method.

Caffeine Standard Spectra