Caffeine HPLC Method

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HPLC method for caffeine analysis

High Pressure Liquid Chromatography Setup

HPLC Pump Gradient Flow Settings
HPLC Pump Solvent Settings
HPLC UV Detector General Settings
HPLC UV Detector Channel 1 Settings
HPLC UV Detector Channel 2 Settings


Results

Caffeine should be detected as sharp peaks at 275nm as can be observed in the image below.

Caffeine Standard Spectra
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