Free Radical Generation
The 1-electron oxidation of a phenol can be accomplished via chemical or enzymatic means.
Chemical Means
1. KMnO4
KMnO4 To make a 2 mM solution of KMnO4, we added 0.03g of KMnO4 solid to 100 mL dH2O
To make a 2 mM solution of BHA, we added 0.0304 g of solid HPA to 100 mL dH2O
2. Cerium (IV)
For Cerium Free Radicals: 100 mL solution of 2 mM HPA and 100 mL solution of Ce (IV).
For 100 mL of 2 mM HPA:
Weigh out 30.4mg of HPA
Add it into 100 mL DI water
For 100 mL of 2 mM Ce (IV)
Weigh out 109.6 mg of Ammonium Cerium Nitrate
Add that to 2.5 mL of 18M sulfuric acid
Add that solution to 97.5 mL of DI water