Ian Salveson Chem430 F16

Chemistry/Biochemistry Research 430

Fall 2016

Ian Salveson

Senior Chemistry Major

Research Times

M/W/F 10am-12pm

Proposed Research Project

Investigating the metabolism of acetaminophen modeled by an enzymatic, single-electron oxidation using HRP.

Project Title

Characterization and Analysis of Acetaminophen Metabolites

General Information

Advisor: Dr. Bradley E. Sturgeon

Proposal

Synthesize the primary products of this oxidation and investigate their activity in a similar system to determine the level to which acetaminophen is oxidized by this type of enzyme.

Instruments to be used

HPLC, Flash Chromotography System, NMR, Immobilized Enzyme column, ESR

References (2 minimum)

1.) Hinson, Jack A., Dean W. Roberts, and Laura P. James. "Mechanisms of Acetaminophen-Induced Liver Necrosis." Handbook of Experimental Pharmacology Adverse Drug Reactions (2009): 369-405. Web. 2.) Potter, David W., Dwight W. Miller, and Jack A. Hinson. "Identification of Acetaminophen Polymerization Products Catalyzed by Horseradish Peroxidase." Journal of Biological Chemistry 260.22 (1985): 12174-2180. Print.

Research pledge

I, Ian Salveson, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.