Louis Badang Chem430 F16

Chemistry/Biochemistry Research 430

Fall 2016

Styles Bitchly

Junior Biochemistry Major

Research Times

T/Thur 2-4 pm

section 01 = 0.25 credit = 4 hours per week.

section 02 = 0.50 credit = 8 hours per week.

Proposed Research Project

General Information

Advisor: Laura Moore

Other research student collaborators: Sobhi Kazmouz

Other Research Collaborators:

Proposal

The salt bridge interaction between Asp 130 and Arg 140 is essential for the dimerization of FNR. In this research, I will produce a double mutant FNR where Asp 130 will be changed into Arg and Arg 140 into Glu at the same time. This is to show definitely that the salt bridge is important to the FNR to dimerize in the absence of oxygen. Also, another mutation that may be done is Arg to Asp 140 mutation to check whether the lack of compatibility in activity values of both mutant FNR samples is caused by the distinction between the size of the R group of the aspartate and glutamate residue.

Research pledge

I, Louis J Badang, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.