N-Acetyl L-Tyrosine

This page depicts the oxidation of N-Acetyl L-Tyrosine (NAT) by Horse Radish Peroxidase (HRP)

NAT

The Dioxane Conundrum

The following two graphs depict the difference in reaction when dioxane is present and when it is not.

Figure 1: NAT oxidation in solution with dioxane and reacted with H2O2 and HRP 6/5/16. This graph shows the dioxane in the solution inhibiting the reaction completely.

Figure 2: 2mM NAT oxidation without dioxane and reacted with 2mM H2O2 and HRP in a beaker 6/5/16

Beaker Reaction vs. Immobilized Enzyme Bio-Reactor

Utilizing the immobilization technique in a bio-reactor allows for the control of the rate of flow of the reaction. This makes the peaks in the products much more defined.

Figure 2: 2mM NAT oxidation without dioxane and reacted with 2mM H2O2 and HRP in a beaker 6/5/16

Figure 3: 2mM NAT oxidation without dioxane and reacted with 1mM H2O2 using the Immobilized Enzyme technique 6/9/16

Concentration Test

Figure 4: 2mM NAT oxidation without dioxane and reacted with 1mM H2O2 and 2mM H202 with immobilized HRP at 0.5ml/min and 1.0ml/min 6/9/16

Figure 5: 10mM NAT oxidized with 5, 10, and 20mM H2O2 with immobilized HRP at 0.5ml/min 6/23/16

Flash Chromatography

Figure 6: Flash chromatography separation of Figure 5 6/28/16