This page depicts the oxidation of N-Acetyl L-Tyrosine (NAT) by Horse Radish Peroxidase (HRP)
The Dioxane Conundrum
The following two graphs depict the difference in reaction when dioxane is present and when it is not.
Figure 1: NAT oxidation in solution with dioxane and reacted with H2O2 and HRP 6/5/16. This graph shows the dioxane in the solution inhibiting the reaction completely.
Figure 2: 2mM NAT oxidation without dioxane and reacted with 2mM H2O2 and HRP in a beaker 6/5/16
Beaker Reaction vs. Immobilized Enzyme Bio-Reactor
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Figure 2: 2mM NAT oxidation without dioxane and reacted with 2mM H2O2 and HRP in a beaker 6/5/16
Figure 3: 2mM NAT oxidation without dioxane and reacted with 1mM H2O2 using the
Immobilized Enzyme technique 6/9/16
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Figure 4: 2mM NAT oxidation without dioxane and reacted with 1mM H2O2 and 2mM H202 with immobilized HRP at 0.5ml/min and 1.0ml/min 6/9/16
Figure 5: 10mM NAT oxidized with 5, 10, and 20mM H2O2 with immobilized HRP at 0.5ml/min 6/23/16
Figure 6: Flash chromatography separation of Figure 5 6/28/16
