Fatty Acid Methyl Esters

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Fatty Acid Methyl Ester Structures

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Significance of Fatty Acid Methyl Esters

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Transesterification

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Image . This reaction is known as transesterification. The methyl from the methanol group is added to the carboxylic acid of the fatty acid to create a fatty acid methyl ester.

Sample Preparation

  • Combined 200 ul of the sample with 40 ul of anhydrous methanol into a microcentrifuge tube
  • Added 4.3 ul of 5 M KOH to the top methanol layer (catalyst)
  • Sonicated the tubes in an ultrasonic bath for 30 minutes
  • Added 200 ul of 0.1 M acetic acid (neutralize the base catalyst)
  • Centrifuged the solution at 6,000 rpm for 5 minutes
  • Transferred 40 ul of the top layer to a GC vial and diluted with pentane

GC-MS Analysis

GC-MS Method for the Analysis of Fatty Acid Methyl Esters:

Grapeseed Oil Data

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Corn Oil Data

Sunflower Oil Data

References

Lipid Residue Analysis of Archaeological Pottery: An Introductory Laboratory Experiment in Archaeological Chemistry Clare S. Harper, Faith V. Macdonald, and Kevin L. Braun Journal of Chemical Education 2017 94 (9), 1309-1313 DOI: 10.1021/acs.jchemed.7b00225

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