Lignan Purification
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Lignan Purification
Solutions of 5 HMR Lignan capsules (0.785 g) and 100.0 mL of EtOH and 10 HMR Lignan capsules (1.629 g) and 100.0 mL of EtOH were prepared. These solutions were filtered and analyzed using the HPLC at absorbance wavelength of 270 nm and 28℃ using an acetonitrile (ACN) and 0.1% TFA gradient for 30 minutes. The first 15 minutes were run at 100% 0.1% TFA, 15-25 minutes at 100% ACN, and 25-30 minutes at 100% 0.1% TFA.