HPA Project
Project Overview
For this project, the goal was to quantify the concentration of HPA remaining in solution after a reaction between hydrogen peroxide, horseradish peroxidase, and HPA is completed. To do this we made standards of known concentrations of HPA, made reaction vials of the hydrogen peroxide, enzyme (horseradish peroxidase), and HPA. We ran these through the HPLC to collect the data.
My research partner, Will Fox, ran a similar experiment with acetaminophen, the link to his page can be found here.
Making the Samples
This project required HPA standards to be made. The following link describes this process.
Instrumentation
After making the standards, we tested the standards using the HPLC to see where the peaks were for the different concentrations of HPA.
The following link goes into more depth about working with the HPLC:
What the HPLC Tells Us
We ran these samples through the HPLC at the wavelength of 273 nm, which is the lambda max for caffeine. Running the samples at this wavelength tells us how much the samples absorb at this wavelength.