SAHounsve Spring 2018

Chemistry/Biochemistry Research 430

Spring 2018

Selene Hounsve

Senior Biochemistry Major

Research Times

Wed: 11AM-2PM

Fri: 4-5 PM

Proposed Research Project

Project Title

Enzymatic Metabolism of Eugenol

General Information

Advisor: Dr. Bradley E. Sturgeon

Proposal

The purpose of the experiment is to report the observation of radical products of eugenol produced by horseradish peroxidase (HRP) and hydrogen peroxide by using HPLC.

Instruments to be used

HPLC and UV-Vis

Experimental

Eugenol standard for HPLC: 15.5 ul of eugenol in 0.1 mM PBS with 10% ethanol, pH 7.4 and 1% tween. Used method "eugenol_SH_020918_b".

Oxidation reaction: 1. 1 mM Eugenol stock substrate of pH 7.4 PBS with 10% ethanol 2. 0.25 M H2O2 Stock 3. HRP Stock

Research pledge

I, Selene Hounsve, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.