Free Radical Generation
The 1-electron oxidation of a phenol can be accomplished via chemical or enzymatic means.
Chemical Means
1) KMnO4
2. Cerium (IV)
For Cerium Free Radicals: 100 mL solution of 2 mM HPA and 100 mL solution of Ce (IV). For 100 mL of 2 mM HPA: Weigh out 30.4mg of HPA Add it into 100 mL DI water For 100 mL of 2 mM Ce (IV) Weigh out 109.6 mg of Ammonium Cerium Nitrate Add that to 2.5 mL of 18M sulfuric acid Add that solution to 97.5 mL of DI water