Ahmad Pauzi

Enzymatic Oxidation of Methyleugenol

Methyleugneol is a phenolic type of flavourant found in essential oils, which is primarily derived from cloves. This compound has been known to be a human carcinogen listed on the National Toxicology Program Report. Evidence suggests that Eugenol displays an extensive span of biological activities.Furthermore, studies has shown that Eugenol to be carcinogenic after long term dietary administration in animals at high doses. The mechanism of hepatic carcinogenesis resulted from the bioactivation of the Methyleugenol has also shown to occur in the liver. The mechanism behind the chemical oxidation of Eugenol is that it gives a one electron oxidation to form a Eugenol radical and the radical will recombine together in a couple of different ways to form polymers. In this study, we show that Eugenol undergoes Enzymatic oxidation with Hydrogen Peroxide and Horseradish peroxidase to form free radicals.