Immobilized Enzymes

The immobilization procedure can be found here.

The procedure has been applied to the following:

HRP/H2O2/TCP

Revisiting the Peroxidase Oxidation of 2,4,6-Trihalophenols: ESR Detection of Radical Intermediates.

Bradley E. Sturgeon,*, Benjamin J. Battenburg, Blake J. Lyon, and Stefan Franzen.

Chemical Research in Toxicology, 2011, 24, pp. 1862-1868.

here

Sturgeon, 2011

HRP/H2O2/tyrosine/NO

Tyrosine iminoxyl radical formation from tyrosyl radical/nitric oxide and nitrosotyrosine.

Sturgeon BE, Glover RE, Chen YR, Burka LT, and Mason RP.

Journal of Biological Chemistry, 2001, 276(49), pp. 45516-45521.

here

Sturgeon, 2001

Cyt c

Protein Oxidation of Cytochrome c by Reactive Halogen Species Enhances Its Peroxidase Activity.

Yeong-Renn Chen, Leesa J. Deterding, Bradley E. Sturgeon, Kenneth B. Tomer, and Ronald P. Mason

Journal of Biological Chemistry, 2002, 277(33), pp. 29781-29791.

here

Chen, 2002