Nitrate in the Freshwater Fish tank

Introduction

Nitrates in surface water primarily result from the decay of autotrophs and from chemoheterotrophic life, for nitrates are a byproduct of the waste produced from the consumption of organic molecules as an energy source. Fish are the primary chemoheterotrophs in aquatic ecosystems; therefore, they are accountable for the majority of nitrogen found in water. Ammonia is the main component of fish waste, which is a very toxic compound of nitrogen, but aerobic ammonia oxidizers convert ammonia to nitrite via the intermediate hydroxylamine, which requires aid from ammonia monooxygenase and hydroxylamine dehydrogenase (2). Nitrite is an even more toxic form of nitrogen, but this is further oxidized to nitrate via another type of nitrogen bacteria with the enzyme nitrite oxidoreductase(2). In nature, nitrates are regulated to a certain extent through aquatic plant life, for it is essential in the production of chlorophyll (). In fish tanks, however, algae is the only natural nitrate regulator, but unlike more complex and nutrient dependent plants, algae is able to grow beyond the capacity of a given ecosystem with ease. Algae cannot convert nitrogen to a gas that can escape the system, so when it dies, nitrogen is released back into the system (). In closed systems, like that of a fish tank, mechanical work such as changing out volumes of water, being mindful in not overfeeding the fish, and cleaning off the algae and filtration systems, is required in order to ensure nitrate levels are kept at a safe and constant concentration. To investigate the outcome of partial water changes on the nitrate and nitrite levels in an freshwater aquarium containing convict cichlids, a consistent feeding plan was devised to ensure the differing nitrate levels in the water are not a result of a fluctuating diet or of decomposing food.

"Outside" surface water contaminants: Though natural regulation proves to be useful in open surface water systems, there are a lot more variables in how nitrates and nitrites can enter the system, therefore, . Nitrate and nitrite levels in surface and ground water can result from agricultural runoff (fertilizers), improper disposal of human and animal waste, and...() The EPA has set different guidelines for surface water that is just a reservoir for aquatic life and water that is used for human consumption. The maximum contaminant level for nitrates and nitrites in drinking water in the U.S.A. are 10.0 ppm and 1.0 ppm respectively (). For water systems not being used as drinking water or connected to..., such as isolated ponds, the EPA recommends that nitrate levels do not exceed 60 ppm and that nitrite levels should be kept as close to 0.0 ppm as possible ().

In addition to nitrates and nitrites, chlorides, phosphates and sulfates should be well monitored in drinking water and surface water. Road-salt runoff, which primarily consists of NaCl, into streams and rivers can pose a major threat to surface water quality and aquatic life. Even in warm weather months, when roads are not being salted, chloride levels from winter salt applications persisted in a study carried out in Milwaukee Massachusetts (1).

Materials and Methods

Fish Tank Set-Up

The closed system for which data was collected consisted of a 39 gallon freshwater fish tank with convict cichlids. An Aquatop Canister Filter was connected to the fish tank via an auto-top-off... A feeding mechanism with the Zacro Automated Fish Feeder was devised so that 1.0 gram of TetraCichlid Floating Cichlid Pellets would be delivered twice a day. This was done by setting the automated fish feeder to release 1 meal portion at 8:30 and 16:30

Fish Tank Samples

In order to see how mainly nitrate and nitrite levels change from day to day in a closed system, five water samples were collected throughout the week at roughly the same time each day (14:00). These samples were then run on the Thermo Fisher Scientific Chromeleon 7 Chromatography Data System, with nitrate, nitrite, chloride, and sulfate calibration standards of 10, 40, and 100 ppm.

Open System Comparison

Freshwater samples from a creek and a pond were collected and analyzed on...from the locations displayed in figure 1.

References

1. Corsi, S. R.; Graczyk, D. J.; Geis, S. W.; Booth, N. L.; Richards, K. D. A Fresh Look at Road Salt: Aquatic Toxicity and Water-Quality Impacts on Local, Regional, and National Scales. Environmental Science & Technology 2010 44,7376-7382.

2. Harms, G.; Layton, A. C.; Dionsi, H. M.; Gregory, I. R.; Garrett, V. M.; Hawkins, S. A.; Robinson, K. G.; Slayer, G. S. Real-Time PCR Quantification of Nitrifying Bacteria in a Municipal Wastewater Treatment Plant. Am. Chem. Soc. 2002, 37, 343-351.