Ovoperoxidase from Sea Urchins
Project Overview
- Urchin9b.jpg
Sea Urchin Egg (02/27/2019)
The Sea Urchins
Gulf Specimen Marine Laboratories is the vendor through which we purchased the sea urchins for research. We purchased around 12 fertilized sea urchins, and were able to collect eggs from one sea urchin out of the first batch.
Below is a link to the website from which we ordered the sea urchins:
Gulf Specimen Marine Laboratories
The following images are of the sea urchin eggs we abstracted.
Sea Urchin Egg (02/27/2019)
Enhanced Image of Sea Urchin Egg (02/27/2019)
Egg Extraction
0.5 mL of 0.55 M KCl was injected into the sea urchins to cause them to release their eggs/sperm. 0.25 mL was injected into either side of the mouth. After the KCl was injected, the urchins were overturned on a beaker to release the eggs/sperm.
The first round of sea urchins had only 4 females.
The following video was used as references to guide our lab experience.
Our Lab Experience
Injecting the urchins with KCl
A sea urchin releasing sperm
Sea urchin sperm release
Sea urchin sperm release
A sea urchin releasing eggs
Sea urchin eggs
Sea urchin spine
Sea urchin egg
Magnified sea urchin egg
.JPG)
Instrumentation
The UV-Vis is the main instrument we use to analyze the peroxidase.
The following link explains the procedure used with the UV-Vis.
References
Scots J-Term Andros Island
January 4th through January 12th of 2019 my research advisor, Brad Sturgeon, along with 7 other students and myself, visited Forfar Field Station on Andros Island in the Bahamas. There I was able to learn about the natural environment of sea urchins We also gained field experience by studying them through snorkeling. The interns at Forfar were very knowledgeable about the area and the creatures inhabiting the ocean around the island.
Below is a link to an article written about the trip as a whole: