Caffeine HPLC Method

From MC Chem Wiki
Revision as of 15:28, 19 April 2016 by Chrisk (talk | contribs) (→‎Results)
Jump to navigation Jump to search

HPLC method for caffeine analysis

High Pressure Liquid Chromatography Setup (HPLC)

HPLC Pump Gradient Flow Settings
HPLC Pump Solvent Settings
HPLC UV Detector General Settings
HPLC UV Detector Channel 1 Settings
HPLC UV Detector Channel 2 Settings

Results

Caffeine should be detected as sharp peaks which elute at approximately 11 minutes when monitoring 275nm on the UV Detector.

The image below is the spectra of a caffeine standard run with the above method.

Caffeine Standard Spectra
Retrieved from "http://205.166.159.208/wiki/index.php?title=Caffeine_HPLC_Method&oldid=879"