Chemical, Enzymatic, and Electrochemical Oxidation of Biophenols

Introduction

• • will add background information about the phenols I am working with. The redox properties of these phenols can be studied using chemical, enzymatic, and electrochemical methods. **will discuss HRP and KMnO4 methods.

This project aims to enzymatically and chemically oxidize HPA with the methods described previously. Whereas chemical and enzymatic methods have a fixed oxidation potential, electrochemical methods allow for control of the oxidation potential. While HPA and N acetyl tyrosine have oxidation potentials capable of being reached by HRP, this is not the case for diiodotyrosine, whose oxidation potential lies outside this range. For diiodotyrosine, cyclic voltammetry studies coupled with subsequent bulk electrolysis will be utilized instead. The oxidation products will be monitored with HPLC and isolated via flash chromatography. Oxidation products with be characterized with HNMR and HR-MS.

Page History

This page was created by Sara L. Simonson in the fall of 2021