Immobilized Enzymes
The immobilization procedure can be found here.
The procedure has been applied to the following:
HRP/H2O2/TCP
- Revisiting the Peroxidase Oxidation of 2,4,6-Trihalophenols: ESR Detection of Radical Intermediates.
- Bradley E. Sturgeon,*, Benjamin J. Battenburg, Blake J. Lyon, and Stefan Franzen.
- Chemical Research in Toxicology, 2011, 24, pp. 1862-1868.
- here
HRP/H2O2/tyrosine/NO
- Tyrosine iminoxyl radical formation from tyrosyl radical/nitric oxide and nitrosotyrosine.
- Sturgeon BE, Glover RE, Chen YR, Burka LT, and Mason RP.
- Journal of Biological Chemistry, 2001, 276(49), pp. 45516-45521.
- here
Cyt c
Protein Oxidation of Cytochrome c by Reactive Halogen Species Enhances Its Peroxidase Activity.
- Yeong-Renn Chen, Leesa J. Deterding, Bradley E. Sturgeon, Kenneth B. Tomer, and Ronald P. Mason
- Journal of Biological Chemistry, 2002, 277(33), pp. 29781-29791.
- here