Fatty Acid Methyl Esters
Background Information
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Significance of Fatty Acid Methyl Esters
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Transesterification
Image . This reaction is known as transesterification. The methyl from the methanol group is added to the carboxylic acid of the fatty acid to create a fatty acid methyl ester.
Sample Preparation
- Combined 200 ul of the sample with 40 ul of anhydrous methanol into a microcentrifuge tube
- Added 4.3 ul of 5 M KOH to the top methanol layer (catalyst)
- Sonicated the tubes in an ultrasonic bath for 30 minutes
- Added 200 ul of 0.1 M acetic acid (neutralize the base catalyst)
- Centrifuged the solution at 6,000 rpm for 5 minutes
- Transferred 40 ul of the top layer to a GC vial and diluted with pentane
GC-MS Analysis
GC-MS Method for the Analysis of Fatty Acid Methyl Esters:
Grapeseed Oil Data
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