HPLC
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The HPLC, or High Pressure Liquid Chromatography instrument, was used to test the caffeine standards and samples.
Description
The following video is a very helpful short introduction to how the HPLC works.
Procedure
Data
Below is the data we analyzed from the HPLC. These chromatograms show where each standard absorbed at 237 nm, so the coffee samples can be tested against these standards for caffeine levels.