Difference between revisions of "SAHounsve Spring 2018"
| Line 26: | Line 26: | ||
Oxidation reaction: | Oxidation reaction: | ||
| − | 1. Eugenol stock substrate of | + | 1. 1 mM Eugenol stock substrate of pH 7.4 PBS with 10% ethanol |
2. 0.25 M H2O2 Stock | 2. 0.25 M H2O2 Stock | ||
3. HRP Stock | 3. HRP Stock | ||
Revision as of 23:30, 10 May 2018
Chemistry/Biochemistry Research 430
Spring 2018
Selene Hounsve
Senior Biochemistry Major
Research Times
Wed: 11AM-2PM
Fri: 4-5 PM
Proposed Research Project
Project Title
Enzymatic Metabolism of Eugenol
General Information
Advisor: Dr. Bradley E. Sturgeon
Proposal
The purpose of the experiment is to report the observation of radical products of eugenol produced by horseradish peroxidase (HRP) and hydrogen peroxide by using HPLC.
Instruments to be used
HPLC and UV-Vis
Experimental
Eugenol standard for HPLC: 15.5 ul of eugenol in 0.1 mM PBS with 10% ethanol, pH 7.4 and 1% tween. Used method "eugenol_SH_020918_b".
Oxidation reaction: 1. 1 mM Eugenol stock substrate of pH 7.4 PBS with 10% ethanol 2. 0.25 M H2O2 Stock 3. HRP Stock
Results
Discussion
Conclusion
References
Research pledge
I, Selene Hounsve, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.