Difference between revisions of "Caffeine HPLC Method"
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| Line 9: | Line 9: | ||
**0.01% Acidified Water | **0.01% Acidified Water | ||
| − | Samples should be filtered | + | Samples should be filtered through 45 μm syringe filters into HPLC sample vials and loaded into the HPLC auto-sampler carousel. |
| + | |||
| + | Trial Configuration | ||
Once prepared, the samples can be injected in 10 μl increments and trials can range in length from 15-30 minutes depending upon the complexity of one's sample. | Once prepared, the samples can be injected in 10 μl increments and trials can range in length from 15-30 minutes depending upon the complexity of one's sample. | ||
Revision as of 15:36, 19 April 2016
Caffeine can be detected using Reversed Phase High Pressure Liquid Chromatography with UV Detection.
In order to accomplish caffeine analysis the HPLC system requires the following:
- C18 column
- UV Detector Monitoring 275nm
- Mobile Phase
- Acetonitrile
- 0.01% Acidified Water
Samples should be filtered through 45 μm syringe filters into HPLC sample vials and loaded into the HPLC auto-sampler carousel.
Trial Configuration Once prepared, the samples can be injected in 10 μl increments and trials can range in length from 15-30 minutes depending upon the complexity of one's sample.
High Pressure Liquid Chromatography Setup (HPLC)
Results
Caffeine should be detected as sharp peaks which elute at approximately 11 minutes when monitoring 275nm on the UV Detector.
The image below is the spectra of a caffeine standard run with the above method.
