Difference between revisions of "SAHounsve Spring 2018"
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| Line 21: | Line 21: | ||
===Experimental=== | ===Experimental=== | ||
| + | Eugenol standard for HPLC: | ||
| + | 15.5 ul of eugenol in 0.1 mM PBS with 10% ethanol, pH 7.4 and 1% tween. Used method "eugenol_SH_020918_b". | ||
| + | |||
| + | Oxidation reaction: | ||
| + | 1. Eugenol stock subtrate of 1.0 mM PBS pH 7.4 and 10% ethanol | ||
| + | 2. 0.25 M H2O2 Stock | ||
| + | 3. HRP stock | ||
| + | |||
===Results=== | ===Results=== | ||
===Discussion=== | ===Discussion=== | ||
Revision as of 14:13, 22 March 2018
Chemistry/Biochemistry Research 430
Spring 2018
Selene Hounsve
Senior Biochemistry Major
Research Times
Wed: 11AM-2PM
Fri: 4-5 PM
Proposed Research Project
Project Title
General Information
Advisor: Dr. Bradley E. Sturgeon
Proposal
To understand the oxidation properties of eugenol by utilizing HPLC.
Instruments to be used
HPLC
Experimental
Eugenol standard for HPLC: 15.5 ul of eugenol in 0.1 mM PBS with 10% ethanol, pH 7.4 and 1% tween. Used method "eugenol_SH_020918_b".
Oxidation reaction: 1. Eugenol stock subtrate of 1.0 mM PBS pH 7.4 and 10% ethanol 2. 0.25 M H2O2 Stock 3. HRP stock
Results
Discussion
Conclusion
References
Research pledge
I, Selene Hounsve, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.