Brittney Book Chem430 F16

Chemistry/Biochemistry Research 430

Fall 2016

Brittney Book

Junior Biochemistry Major

Research Times

T/Thur 2-4 pm

section 01 = 0.25 credit = 4 hours per week.

section 02 = 0.50 credit = 8 hours per week.

Proposed Research Project

General Information

Advisor: Audra Sostarecz

Other research student collaborators: Ben, Kelly, Nichole Musselman, and Izabela Sartori

Other Research Collaborators: Colorado State University, Prof. Debbie Crans

Abstract

Langmuir-Blodgett Monolayers of propolis and phospholipids can be examined for antibacterial and anti-cancerous properties.  The Langmuir Monolayer technique allows for the analysis of the organization of amphiphilic molecules at an air-water interface and is, therefore, a useful technique for the formation of model cell membranes. What is propolis? Propolis is a green-yellow to red-brown resinous material collected from various vegetation around the hive and used to cover the walls of the hive, keep out intruders, and keep out harmful pathogens. There are different chemical composition of the propolis based on the vegetation at the geological location. As a result the biological activity of the propolis is related to the plants native to the site of collection (toreti, 2013).  Langmuir monolayers of American propolis are found to have a higher degree of order (less fluidity) and to be more stable indicated by a higher surface pressure at low molecular areas when incorporated into a film with phospholipids such as dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE).

Instruments to be used

Kibron Trough

References (2 minimum)

Toreti, V. C., Sato H. H., Pastore G. M., & Park Y. K. (2013). Recent Progress of Propolis for Its Biological and Chemical Compositions and Its Botanical Origin. Review Article, 2013, 13.

Naramoto K., Massashi K., & Ichihara K. (2014). Effects of an Ethanol Exact of Brazilian Green Propolis on Human Cytochrome P450 Enzyme Activities in Vitro. Journal of Agricultural and Food Chemistry, 2014, 62.

Savka M. A., Dailey L., Popova M., Mihaylova R., Merritt B., Masek M., Le P., Nor S. R., Ahmad M., Hudson A. O., & Bankova V. (2015).Chemical Composition and Disruption of Quorum Sensing Signaling in Geographically Diverse United States Propolis. Review Article, 2015.

Research pledge

I, Brittney Book, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.