Difference between revisions of "N-Acetyl L-Tyrosine"

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==alskhdlahksg==
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==Concentration Test==
  
 
[[File:NEW69.jpg|500px|thumb|left|Figure 4:  2mM NAT oxidation without dioxane and reacted with 1mM H2O2 and 2mM H202 with immobilized HRP at 0.5ml/min and 1.0ml/min 6/9/16]]
 
[[File:NEW69.jpg|500px|thumb|left|Figure 4:  2mM NAT oxidation without dioxane and reacted with 1mM H2O2 and 2mM H202 with immobilized HRP at 0.5ml/min and 1.0ml/min 6/9/16]]
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[[File:KD9.jpg|500px|thumb|none|Figure 5: 10mM NAT oxidized with 5, 10, and 20mM H2O2 with immobilized HRP at 0.5ml/min 6/23/16]]
 
[[File:KD9.jpg|500px|thumb|none|Figure 5: 10mM NAT oxidized with 5, 10, and 20mM H2O2 with immobilized HRP at 0.5ml/min 6/23/16]]
  
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==Flash Chromatography==
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[[File:DK 11.jpg|500px|thumb|left|Figure 6: Flash chromatography separation of Figure 5 6/28/16]]
 
[[File:DK 11.jpg|500px|thumb|left|Figure 6: Flash chromatography separation of Figure 5 6/28/16]]

Revision as of 17:36, 29 June 2016

This page depicts the oxidation of N-Acetyl L-Tyrosine (NAT) by Horse Radish Peroxidase (HRP)

NAT

The Dioxane Conundrum

The following two graphs depict the difference in reaction when dioxane is present and when it is not.

Figure 1: NAT oxidation in solution with dioxane and reacted with H2O2 and HRP 6/5/16. This graph shows the dioxane in the solution inhibiting the reaction completely.
Figure 2: 2mM NAT oxidation without dioxane and reacted with 2mM H2O2 and HRP in a beaker 6/5/16


Beaker Reaction vs. Immobilized Enzyme Bio-Reactor

Utilizing the immobilization technique in a bio-reactor allows for the control of the rate of flow of the reaction. This makes the peaks in the products much more defined.

Figure 2: 2mM NAT oxidation without dioxane and reacted with 2mM H2O2 and HRP in a beaker 6/5/16
Figure 3: 2mM NAT oxidation without dioxane and reacted with 1mM H2O2 using the Immobilized Enzyme technique 6/9/16



Concentration Test

Figure 4: 2mM NAT oxidation without dioxane and reacted with 1mM H2O2 and 2mM H202 with immobilized HRP at 0.5ml/min and 1.0ml/min 6/9/16
Figure 5: 10mM NAT oxidized with 5, 10, and 20mM H2O2 with immobilized HRP at 0.5ml/min 6/23/16


Flash Chromatography

Figure 6: Flash chromatography separation of Figure 5 6/28/16