Difference between revisions of "HPA Reactions"
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[[File:4-Hydroxyphenylacetic acid.svg.png|200px|thumb|none|HPA]] | [[File:4-Hydroxyphenylacetic acid.svg.png|200px|thumb|none|HPA]] | ||
| − | + | ==Product Detection Test== | |
Reaction of 50/50 HPA-Dioxane Solution in Buffer with various H202 concentrations and Horse Radish Peroxidase (HRP) | Reaction of 50/50 HPA-Dioxane Solution in Buffer with various H202 concentrations and Horse Radish Peroxidase (HRP) | ||
[[File:Layout HPA 02.jpg|500px|thumb|Left|HPA/H202/HPA (6/3/16)]] | [[File:Layout HPA 02.jpg|500px|thumb|Left|HPA/H202/HPA (6/3/16)]] | ||
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[[File:IE HPA5 02.jpg|500px|thumb|none|IE HRP HPA/H202 (6/15/16)]] | [[File:IE HPA5 02.jpg|500px|thumb|none|IE HRP HPA/H202 (6/15/16)]] | ||
| − | + | ==Concentration Test== | |
To increase the amount of product extractable, the concentration of HPA was increased to 50mM as well as the H202 concentration, however the same amount of products does not appear as well as the formation of polymers that block products at the 0.25mL/Min run of the immobilized enzyme. | To increase the amount of product extractable, the concentration of HPA was increased to 50mM as well as the H202 concentration, however the same amount of products does not appear as well as the formation of polymers that block products at the 0.25mL/Min run of the immobilized enzyme. | ||
[[File:IE HPA7 02.jpg|500px|thumb|Left|IE HRP HPA/H202 (6/20/16)]] | [[File:IE HPA7 02.jpg|500px|thumb|Left|IE HRP HPA/H202 (6/20/16)]] | ||
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[[File:IE HPA9 02.jpg|500px|thumb|Left|IE HRP HPA/H202 (6/23/16)]] | [[File:IE HPA9 02.jpg|500px|thumb|Left|IE HRP HPA/H202 (6/23/16)]] | ||
[[File:IE HPA9 01.jpg|500px|thumb|none|IE HRP HPA/H202 (6/23/16)]] | [[File:IE HPA9 01.jpg|500px|thumb|none|IE HRP HPA/H202 (6/23/16)]] | ||
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| + | ==Flash Chromatography Separation== | ||
| + | To try to collect a cleaner sample of the products created in the HPA + H2O2 + HRP reaction, Flash chromatography was preformed to separate the peaks. | ||
| + | |||
| + | This is an HPLC of the separation preformed by the Flash Chromatography instrument on the 0.25mL/min 20mM HPA run from 6/23/16. However, the separation of the assumed three product peaks is not clear. | ||
| + | [[File:IE HPA11 04.jpg|500px|thumb|Left|IE HPA Flash Results (6/27/16)]] | ||
| + | [[File:IE HPA11 03.jpg|500px|thumb|none|IE HPA Flash Results (6/27/16)]] | ||
Revision as of 16:17, 29 June 2016
4-Hydroxyphenylacetic acid (HPA)
Product Detection Test
Reaction of 50/50 HPA-Dioxane Solution in Buffer with various H202 concentrations and Horse Radish Peroxidase (HRP)
Using the Immobilized Enzyme technique, a reaction of HPA-Dioxane and H202 with Immobilized HRP
Trying out different flow speeds with IE to try and see different products. With the greater absorption of HPA There is only a slight change in the product of the reactions
Concentration Test
To increase the amount of product extractable, the concentration of HPA was increased to 50mM as well as the H202 concentration, however the same amount of products does not appear as well as the formation of polymers that block products at the 0.25mL/Min run of the immobilized enzyme.
Decreasing the Concentration to just 20mM brought the resurgence of the peaks of products. These peaks will be separated in flash chromatography given that they are high enough in yield to be detected by the instrument.
Flash Chromatography Separation
To try to collect a cleaner sample of the products created in the HPA + H2O2 + HRP reaction, Flash chromatography was preformed to separate the peaks.
This is an HPLC of the separation preformed by the Flash Chromatography instrument on the 0.25mL/min 20mM HPA run from 6/23/16. However, the separation of the assumed three product peaks is not clear.
