Difference between revisions of "Methods include Qiagen DNA isolation kits"
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'''DNA Extraction''' | '''DNA Extraction''' | ||
| − | + | Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell. | |
'''Purification''' | '''Purification''' | ||
| − | + | To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column. | |
'''Wash''' | '''Wash''' | ||
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'''Elution''' | '''Elution''' | ||
| − | |||
==DNeasy PowerWater Sterivex Kit== | ==DNeasy PowerWater Sterivex Kit== | ||
Revision as of 21:55, 15 September 2021
Qiagen Kit Overview
QIAGEN offers molecular sample and assay technology. Some of these technologies are provided as kits. These kits utilize spin columns to isolate DNA and RNA.
DNA Extraction Lysis Buffer breaks down the cell membrane to release intercellular materials like DNA, RNA, and other organelles from the cell. Purification To bind the DNA to the silica spin column, Chaotropic Salts are critical. Salts disrupt Hydrogen bonding between strands and allow the DNA to adhere to the silica column. Wash
Dry
Elution