Acetaminophen Project

Abstract

Acetaminophen(APAP) is an active ingredient in many over-the-counter and prescription painkillers, such as Tylenol and Oxycodone. APAP is also responsible for approximately 50% of the cases of acute liver-failure in the United States and Great Britain(1). Treatments for acetaminophen-induced liver injury(AILI) are limited. The current mechanism for AILI is the production of hepatotoxic NAPQI as a metabolite in an enzymatic, two-electron oxidation(1). However, evidence also supports a one electron oxidation(2). In an effort to reduce the mortality rate of acetaminophen overdose, we are investigating the identity of single-electron oxidation products and their bioactivity as an alternative mechanism for AILI. An oxidation product of APAP was isolated via high performance liquid chromatography (HPLC) and subsequent flash chromatography. The evidence of a radical intermediate was proven via EPR. The dimer was oxidized in order to test bioactivity and a novel product was observed in the resultant chromatography. These oxidation products can be isolated, identified, and investigated further in order to assess bioactivity.

Introduction

Figure 1: The structure of acetaminophen

Acetaminophen (Figure 1), often referred to as "APAP" is an active ingredient in many over-the-counter and prescription painkillers, such as Tylenol and Oxycodone. The mechanism of pain relief has still not been proven thoroughly. The drug works to raise the pain threshold for a person, effectively increasing the body's requirement for more pain signals in order to feel pain. One of the players in pain are prostaglandins. These are lipid molecules that are made at the site of tissue damage that cause inflammation, and inflammation causes pain (3). Prostaglandins do many other physiological jobs such as protecting the lining of the stomach (3). Enzymes that produce prostaglandins are called cyclooxygenases. These enzymes are often the target for analgesics. APAP originally was thought to inhibit prostaglandin formation in the brain, but was later disputed (3). This was due to the finding that APAP does not suppress PG formation in broken cells, but intact cells(3). Thus, APAP is regarded as a weak inhibitor of both COX-1 and COX-2 enzymes. However, APAP does not have a harmful effect on the gastrointestinal tract, does not affect platelet function, and does not cause breathing issues in asthmatics and therefor has been theorized to be be a selective COX-2 inhibitor (3). Further research showed that APAP is 4.4 times more likely to be selective for COX-2 than COX-1(3). Since this is the case, long term usage of APAP should be cautioned due to potential cardiovascular issues (3).

APAP is also responsible for approximately 50% of the cases of acute liver-failure in the United States and Great Britain(1). Treatments for acetaminophen-induced liver injury(AILI) are limited. The current mechanism for AILI is the production of hepatotoxic NAPQI as a metabolite in an enzymatic, two-electron oxidation(1). At the overdose of acetaminophen, glutathione is depleted by binding to NAPQI. When glutathione is depleted 70% or more, NAPQI can no longer be detoxified by that pathway, and the excess of NAPQI binds covalently to cellular protein macromolecules in the liver, causing cell death and hepatic necrosis [4]. Currently, there is only one approved treatment for acetaminophen overdose and that is a compound called N-acetylcysteine (NAC). Administration of NAC (<8 hours after ingestion) is crucial for decreasing the risk of hepatotoxicity. There are several side effects after administration and caution should be used when dealing with the compound. It is thought to provide cysteine for glutathione synthesis and possibly to form an adduct directly with the toxic metabolite of acetaminophen, N-acetyl-p-benzoquinoneimine [5]. However, this has not been proven in vivo.

Evidence also supports a one electron oxidation(2). A radical intermediate was detected via ESR in the 1980s(6,7). Specifically, a phenoxyl radical was detected and subsequent polymeric material formed(6,7). Thus, this radical chemistry could be forming biologically active products that contribute to hepatotoxicity. The oxidation products were also identified shortly after via nuclear magnetic resonance(2). However, there lacks evidence in the literature regarding isolation and further experimentation of these oxidation products. Therein lies the reason for investigating the identity of single-electron oxidation products and their bioactivity as an alternative mechanism for AILI. In order to determine the identities and properties of the single electron oxidation products, it is necessary to first synthesize them. The single electron oxidation can be accomplished by using a system of horseradish peroxidase (HRP) and hydrogen peroxide. HRP carries out two, single-electron oxidations of the substrate, APAP in this case. Cytochrome P450s (CYPs) metabolize countless fo endogenous and exogenous chemicals in the liver including APAP. CYPs do not bind, oxidize, and move on when encountering substances in the liver. These enzymes behave similar to a peroxidase- such as HRP. The HRP reaction ran in vitro (Figure 2) mimics the reactions that occur in the liver.

Figure 2: The equation for the oxidation of APAP (represented here by AH₂) by HRP and H₂O₂. The (•) denotes a radical.

Pharmacokinetics & Pharmacodynamics

Therapeutic doses in adults range from 1-4 g/day. The minimum lethal dose is 5-15 g, with acute lethal doses ranging from 13-25 g [8]. An Acetaminophen dose of 150 mg/kg causes liver injury. Peak plasma concentrations are seen within 2 hours of ingestion. The half-life is usually 1-3 hours. The metabolites of Acetaminophen stay in the body from 4-5 hours[9]. Hepatotoxicity usually develops 24-36 hours after ingestion, whereas renal insufficiency may develop in 2 to 4 days [10].

Acetaminophen is absorbed from the gastrointestinal tract and exhibits dose-dependent kinetics (first order). Plasma protein binding for Acetaminophen is between 20 and 50%, and passage of the blood-brain barrier is restricted. Elimination of the metabolized drug is though the kidney [10,11].

Metabolism and Excretion

About 90% of acetaminophen is biotransformed by cytochrome P-450 in the liver. Main metabolites are sulphate (about 52%) and glucuronide (about 42%) conjugates. About 4% of the drug is biotransformed to N-acetyl-p-benzoquinoneimine (NAPQI), which is a highly reactive cytotoxic intermediate. NAPQI is detoxified by conjugation with glutathione and excreted in the urine as cysteine and mercapturic acid [10,11].90-100% of an ingested dose is excreted in urine during about 24 h [12]. Approximately 2 % of acetaminophen is excreted unchanged [13].

Figure 3:These are the structures that result after modification by P-450 enzymes in the liver (Sturgeon)

Figure 4:This figure represents the possible pathways in the liver that APAP may take and the percentage of the original molecule that takes each route. (14)

Materials and Methods

Acetaminophen Sample Prep

Early Acetaminophen Trial Sample Preparation

A 2 mM acetaminophen pH 5 stock solution was prepared in 100 mL of nanopure water. Stock solutions of Hydrogen peroxide (0.413 M), and HRP were created. The experimental solutions of acetaminophen (2 mM), H₂)₂ (0.25 M, 0.5 M, 1 M, and 2M), and ten uL of horseradish peroxidase was to each of a reaction vial. (NOTE: this method was to visualize the reaction on the HPLC. In order to obtain a more efficient reaction, the method was updated.)

Recent Acetaminophen Trial Sample Preparation

A 50 mM acetaminophen stock solution was prepared in 100 mL of nanopure water. Stock solutions of Hydrogen peroxide (0.413 M), and HRP were created. The APAP was added to a 1:1 water and dioxane solution. The experimental solutions of acetaminophen (50 mM), H₂O₂ (12.5mM), and ten uL of horseradish peroxidase was to each of a reaction vial. The resulting products were diluted by a factor of 10.

Sample Concentration

The solutions containing our oxidized APAP were spun on a Savant Speed Vac® SC110 with attached Labconco FreeZone 4.5 refrigeration trap for 3 hours until the vials showed a yellow powdery solid at the bottom. The solid was solubilized in lab-grade ethanol (2 mL) in preparation for flash chromatography.

HPLC

Reverse phase high pressure liquid chromatography (HPLC) was performed on an Agilent Technologies 1100 Series instrument using a C18 column. The experimental method consisted of a 30-minute long run with mobile phase beginning at 100 percent acidified water to 100 percent 30% volume to volume acetonitrile. UV/Vis spectroscopy was used as a detector for absorbance at 270 nm.

Flash Chromatography

The solubilized and concentrated products were combined and loaded onto a C18 RediSep R_f gold column. The column was loaded onto a Teledyne Isco CombiFlash R_f 200i instrument. The experiment was 18 minutes long and acetonitrile and acidified water were used. The method was arranged so that the mobile phase started at 100% acidified water and increased linearly until it was 80% acetonitrile and 20% acidified water. Once the peaks were all off the column, the run was terminated and the respective vials were collected.

EPR

A 50 mM solution of APAP pH 5 in 1:1 water to dioxane was made and subsequently diluted by a factor of 10. H₂O₂ (12.5mM) was added to the solution before loading in into a 60 mL syringe. Flow was analyzed at rates of 0.5 mL/minute and 1 mL/minute.

Enzyme Immobilization

An immobilization of peroxidase from horseradish (HRP) was completed using Affi-Gel® 10. This procedure is centered around the isolectric point of the enzyme. The immobilization was completed using a MOPS buffer (pH 7.0) and about 1 mL of Affi-Gel® 10 beads.

Results

Early research efforts to visualize the oxidation of APAP were successful (Figure 5) and four distinct oxidation products were observed as well as a large mass of products representing polymeric material. This aligns with the findings in the literature (2,6,7). The reaction method was adjusted as to concentrate the amount of APAP being oxidized to maximize the amount of product being formed, in addition to solubilizing the products in 1:1 water:dioxane for sufficient HPLC visualization (Figure 6). The "dimer" product was isolated via flash chromatography (Figure 8) and was further oxidized to form a single product shown in Figure 7. The product resulting from this experiment does not align with any of the initial APAP oxidation products, but this provides marginal evidence that the oxidation of APAP does form active compounds. Efforts were made to recreate previous EPR results from Sturgeon, however the data was inconclusive. Computational chemistry using "Web MO/Gaussian" simulated an EPR experiment (Figure 10; Figure 11) using the isotropic fermi contact couplings generated by the software and a pattern similar to the Sturgeon data can be observed. The software was also used to generate visual aids of where the radical can be located on the radicalized APAP molecule (Figure 12; Figure 13). Figure 13 shows that the radical localizes to the ortho positions on the APAP molecule. ΔH_f calculations for the radical reaction on the phenoxyl were made using the values provided by the "Web MO/Gaussian" calculations using B3LYP/6-311+G(2d,p) basis sets. The results of a geometry optimization are given in Figure 11. A value of 1012.59 kJ/mol was found.

HPLC

Figure 5: 2mM APAP was oxidized by increasing amounts of H₂O₂ in the presence of HRP

Figure 6: Oxidation of acetaminophen performed in 1:1 dioxane to water.

Figure 7: 50 mM APAP was oxidized and the first product peak (dubbed "the dimer") was collected via flash chromatography. The dimer was oxidized via HRP and H2 O2 and the resulting product peak eluted

Flash Chromatography

Figure 8: This chromatogram elucidates which vial will contain the desired product. In this case, vials 13 & 14 were collected and combined in order to give rise to the "dimer" peak shown in the chromatography above.

EPR

Figure 9: Experimental acetaminophen oxidation EPR data along with WINSIM simulation (Sturgeon)

Figure 10: WINSIM simulation using the hyperfine coupling constants found after performing a WebMO calculation

B3YLP/6-311+G(2d,p)

Figure 11: Results of a geometry optimization of the radicalized acetaminophen ran using B3YLP/6-311+G(2d,p) basis set

Figure 12: This molecule is an acetaminophen radical. The hydrogen bond was eliminated off of the phenoxy.

Figure 13: Electron Density map: the green/blue areas are the electron dense areas; the electrons spend the most time here

Phenoxy (G)
2.04
6.11
5.73
1.71
1.73
1.36

Radical Location

Figure 14: The radical resonance structures are shown.

ΔH_f Calculations

ΔH_rxn = ΔH_f(Products) - ΔH_f(Reactants)

ΔH_rxn = ΔH_f[(-OH•) + (H+)]- H_f(-OH)

ΔH_rxn = (-515.01158420 + -0.08303 Hartree) - (-515.480291398 Hartree)

ΔH_rxn = 0.385677198 Hartree = 1012.59556048 kJ/mol

Discussion

Gepasi

We used the software "Gepasi" to analyze the kinetics of the reaction between horseradish peroxidase (HRP) and hydrogen peroxide. This program simulates how the compounds will react with each other. The following link describes how to use "Gepasi":

Gepasi

References

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14.) Yan, M; Huo, Yazhen; Yin, Shutao; Hu, Hongbu. “Mechanisms of Acetaminophen-Induced Liver Injury and Its Implications for Therapeutic Interventions.” Redox biology. 17 (2018): n. pag 274-283. Web.