Difference between revisions of "Lignan Degradation"

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===Materials and Methods===
 
===Materials and Methods===
Lignans for Life Hydroxymatairesinol (HMR) Lignan was obtained from Amazon.com (Seattle, WA, USA), and pH 5 Phosphate-Citrate Buffer and 1,4-Dioxane and were obtained from Sigma Aldrich (St. Louis, MO, USA).
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Lignans for Life Hydroxymatairesinol (HMR) Lignan was obtained from Amazon.com (Seattle, WA, USA), and pH 5 Phosphate-Citrate Buffer, 1,4-Dioxane, and KMnO<sub>4</sub> and were obtained from Sigma Aldrich (St. Louis, MO, USA).
  
A solution of 10 HMR Lignan pills in 100.0 mL of 1,4-Dioxane solution was prepared. This HMR Lignan/dioxane solution (25.0 mL) was added to 25.0 mL of pH 5 buffer.
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A solution of 10 HMR Lignan pills in 100.0 mL of 1,4-Dioxane solution was prepared. This HMR Lignan/dioxane solution (25.0 mL) was added to 25.0 mL of pH 5 Phosphate-Citrate buffer.
  
 
A solution of 0.0395 g of KMnO<sub>4</sub> and 1000 μL H<sub>2</sub>O was prepared.
 
A solution of 0.0395 g of KMnO<sub>4</sub> and 1000 μL H<sub>2</sub>O was prepared.

Revision as of 21:26, 4 May 2021

Materials and Methods

Lignans for Life Hydroxymatairesinol (HMR) Lignan was obtained from Amazon.com (Seattle, WA, USA), and pH 5 Phosphate-Citrate Buffer, 1,4-Dioxane, and KMnO4 and were obtained from Sigma Aldrich (St. Louis, MO, USA).

A solution of 10 HMR Lignan pills in 100.0 mL of 1,4-Dioxane solution was prepared. This HMR Lignan/dioxane solution (25.0 mL) was added to 25.0 mL of pH 5 Phosphate-Citrate buffer.

A solution of 0.0395 g of KMnO4 and 1000 μL H2O was prepared.

The following solutions were analyzed on an Agilent 1100 HPLC with a C18 column (4.16 x 100 mm length and 3.5 μm particle size) at absorbance wavelength of 270 nm and temperature of 28°C using an acetonitrile (ACN) and 0.1% TFA gradient for 30 minutes. The first 15 minutes were run at 100% 0.1% TFA, 15-25 minutes at 100% ACN, and 25-30 minutes at 100% 0.1% TFA:

  • 5.0 mL of the HMR Lignan/dioxane/buffer solution
  • 5.0 mL of the HMR Lignan/dioxane/buffer solution + 5 mMol KMnO4
  • 5.0 mL of the HMR Lignan/dioxane/buffer solution + 10 mMol KMnO4
  • 5.0 mL of the HMR Lignan/dioxane/buffer solution + 15 mMol KMnO4