Difference between revisions of "Lignan Degradation"
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===Materials and Methods=== | ===Materials and Methods=== | ||
| − | Lignans for Life Hydroxymatairesinol (HMR) Lignan was obtained from Amazon.com (Seattle, WA, USA), and | + | Lignans for Life Hydroxymatairesinol (HMR) Lignan was obtained from Amazon.com (Seattle, WA, USA), and pH 5 Phosphate-Citrate Buffer and 1,4-Dioxane and were obtained from Sigma Aldrich (St. Louis, MO, USA). |
| − | A solution of 10 HMR Lignan pills in 100.0 mL of 1,4-Dioxane | + | A solution of 10 HMR Lignan pills in 100.0 mL of 1,4-Dioxane solution was prepared. This HMR Lignan/dioxane solution (25.0 mL) was added to 25.0 mL of pH 5 buffer. |
A solution of 0.0395 g of KMnO<sub>4</sub> and 1000 μL H<sub>2</sub>O was prepared. | A solution of 0.0395 g of KMnO<sub>4</sub> and 1000 μL H<sub>2</sub>O was prepared. | ||
Revision as of 19:33, 4 May 2021
Materials and Methods
Lignans for Life Hydroxymatairesinol (HMR) Lignan was obtained from Amazon.com (Seattle, WA, USA), and pH 5 Phosphate-Citrate Buffer and 1,4-Dioxane and were obtained from Sigma Aldrich (St. Louis, MO, USA).
A solution of 10 HMR Lignan pills in 100.0 mL of 1,4-Dioxane solution was prepared. This HMR Lignan/dioxane solution (25.0 mL) was added to 25.0 mL of pH 5 buffer.
A solution of 0.0395 g of KMnO4 and 1000 μL H2O was prepared.
The following solutions were run on the HPLC at absorbance wavelength of 270 nm and 28°C using an acetonitrile (ACN) and 0.1% TFA gradient for 30 minutes. The first 15 minutes were run at 100% 0.1% TFA, 15-25 minutes at 100% ACN, and 25-30 minutes at 100% 0.1% TFA:
- 5.0 mL of the HMR Lignan/dioxane/buffer solution
- 5.0 mL of the HMR Lignan/dioxane/buffer solution + 5 mMol KMnO4
- 5.0 mL of the HMR Lignan/dioxane/buffer solution + 10 mMol KMnO4
- 5.0 mL of the HMR Lignan/dioxane/buffer solution + 15 mMol KMnO4