Difference between revisions of "Freshwater Aquarium Microbiome"
| Line 1: | Line 1: | ||
==Introduction== | ==Introduction== | ||
| − | Microbiomes, the communities of microorganisms living together in particular habitats, are vital for maintaining ecological balance. The habitat hosting the microbial communities can be anything from the gastrointestinal tract of a cow to the soils of the land... The fish themselves oftentimes possess more microbial cells than fish cells in their bodies (2) Up until 2012, there have not been very many studies pertaining to the characterization of the microbiome pertaining to the water associated with freshwater ornamental fishes (3) | + | Microbiomes, the communities of microorganisms living together in particular habitats, are vital for maintaining ecological balance. The habitat hosting the microbial communities can be anything from the gastrointestinal tract of a cow to the soils of the land... The fish themselves oftentimes possess more microbial cells than fish cells in their bodies (2) Up until 2012, there have not been very many studies pertaining to the characterization of the microbiome pertaining to the water associated with freshwater ornamental fishes (3)... To accomplish this, a protocol modeled after Smith et al will be established. 600 mL of aquarium water will be filtered through a 0.22 um filter unit. The concentrated microbial biomass stuck on the filter will be treated with a Puregene Qiagen kit with some modifications from the protocol. Amplification of the bacterial 16S rRNA gene will ensue. |
==Materials and Methods== | ==Materials and Methods== | ||
Revision as of 00:32, 3 March 2021
Introduction
Microbiomes, the communities of microorganisms living together in particular habitats, are vital for maintaining ecological balance. The habitat hosting the microbial communities can be anything from the gastrointestinal tract of a cow to the soils of the land... The fish themselves oftentimes possess more microbial cells than fish cells in their bodies (2) Up until 2012, there have not been very many studies pertaining to the characterization of the microbiome pertaining to the water associated with freshwater ornamental fishes (3)... To accomplish this, a protocol modeled after Smith et al will be established. 600 mL of aquarium water will be filtered through a 0.22 um filter unit. The concentrated microbial biomass stuck on the filter will be treated with a Puregene Qiagen kit with some modifications from the protocol. Amplification of the bacterial 16S rRNA gene will ensue.
Materials and Methods
Results
Discussion
References
2. Savage DC (1977) Microbial ecology of the gastrointestinal tract. Ann Rev Microbiol 31: 107-133
3. Smith, K. F.; Schmidt, V.; Rosen, G. E.; Amaral-Zettler, L. Microbial Diversity and Potential Pathogens in Ornamental Fish Aquarium Water. Public Library of Science One. 2012. 7.
This page was created by Sara L Simonson in the spring of 2021