Difference between revisions of "Peroxidase Oxidation data Ascorbate"
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'''HRP Catalyzed Reaction''' | '''HRP Catalyzed Reaction''' | ||
| − | 2APAP + H2O2 > 2APAP. > diAPAP + H2O + 1/ | + | 2APAP + H2O2 > 2APAP. > diAPAP + H2O + 1/2 O2 |
| − | 2diAPAP + H2O2 > 2diAPAP. > tetraAPAP + H2O + 1/ | + | 2diAPAP + H2O2 > 2diAPAP. > tetraAPAP + H2O + 1/2 O2 |
And so on... | And so on... | ||
| Line 11: | Line 11: | ||
'''HRP Catalyzed Reaction with Ascorbate''' | '''HRP Catalyzed Reaction with Ascorbate''' | ||
| − | 2diAPAP + H2O2 + Ascorbate > 2diAPAP. + Ascorbate > 2diAPAP + Ascorbate. + H2O + 1/ | + | 2diAPAP + H2O2 + Ascorbate > 2diAPAP. + Ascorbate > 2diAPAP + Ascorbate. + H2O + 1/2 O2 |
Latest revision as of 18:57, 5 February 2021
Ascorbate can be utilized as a radical quencher as it is in vivo. In doing so, it is able to effectively limit the size of the Acetaminophen (APAP) polymer products formed through its reaction with APAP, Horseradish peroxidase (HRP) and variable amounts of H2O2 through the equations given below.
HRP Catalyzed Reaction
2APAP + H2O2 > 2APAP. > diAPAP + H2O + 1/2 O2
2diAPAP + H2O2 > 2diAPAP. > tetraAPAP + H2O + 1/2 O2
And so on...
HRP Catalyzed Reaction with Ascorbate
2diAPAP + H2O2 + Ascorbate > 2diAPAP. + Ascorbate > 2diAPAP + Ascorbate. + H2O + 1/2 O2