Difference between revisions of "Acetaminophen Project"
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'''2 mM Acetaminophen Buffer Stock Solution''' | '''2 mM Acetaminophen Buffer Stock Solution''' | ||
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Put the 2 mM Acetaminophen standard into a jar, and add the pH 5 buffer tablet. Stir until dissolved. | Put the 2 mM Acetaminophen standard into a jar, and add the pH 5 buffer tablet. Stir until dissolved. | ||
'''Hydrogen Peroxide Stock Solution''' | '''Hydrogen Peroxide Stock Solution''' | ||
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Add 5 mL of water into a scintillation vial. Add 283 uL of 30% Hydrogen Peroxide to the water. The solution is now at about 0.413 M. | Add 5 mL of water into a scintillation vial. Add 283 uL of 30% Hydrogen Peroxide to the water. The solution is now at about 0.413 M. | ||
[[File:Aceta_pic1|400px|thumb|left|Figure 1. Graph of the Acetaminophen standards]] | [[File:Aceta_pic1|400px|thumb|left|Figure 1. Graph of the Acetaminophen standards]] | ||
Revision as of 14:52, 20 June 2019
This project's goal was to quantify the concentration of Acetaminophen left after a reaction with hydrogen peroxide, Acetaminophen, & horseradish peroxidase. To do this, I made standards with known concentrations of Acetaminophen. Then, we ran the reaction in three separate vials with varying amounts of peroxide with the constant amounts of Acetaminophen and horseradish peroxidase (HRP).
My research partner, Josie Welker, ran a similar experiment with HPA, the link to his page can be found here.
Acetaminophen Standards
In order for our samples to be tested, a 2 mM Acetaminophen pH 5 solution, Hydrogen peroxide stock solution, and horseradish peroxidase solution were made.
2 mM Acetaminophen Buffer Stock Solution
Put the 2 mM Acetaminophen standard into a jar, and add the pH 5 buffer tablet. Stir until dissolved.
Hydrogen Peroxide Stock Solution
Add 5 mL of water into a scintillation vial. Add 283 uL of 30% Hydrogen Peroxide to the water. The solution is now at about 0.413 M.