Gepasi

Procedure

1) Rename title.

2) Add reactions.

3) Make sure the number of metabolites matches the reactions.

4) Click "kinetics."

5) Choose "mass action (reversible)."

6) Enter k1 and k2 values for each reaction.

7) Choose "metabolite."

8) Enter concentrations for each metabolite.

9) Click "time course" choose run.

To Save Data

1) Click the task tab.

2) Choose "edit."

3) Add metabolites and time.

4) Rename the file time course.

5) Click "run."

Helpful Tips

If your extracted data is not showing points for the entire run time you have selected, increase the number of points in Gepasi under the "Task" tab.

Additionally, if you are putting the data into a wavemetric software like IGOR, and your waves are downloading as 2 dimensional waves, append the waves to the table and edit them from there. Editing in the table will change the waves everywhere in the software.

Example Graphs

Figure 1. Graph of the HRP phases over a time span of 0.02 seconds.

Figure 2. Graph of the HRP phases with the HRP2 rate increased to 1e^5.

Figure 3. Graph of the HRP phases with the HRP2 rate increased to 1e^6.