Difference between revisions of "Caffeine HPLC Method"

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HPLC method for caffeine analysis
 
HPLC method for caffeine analysis
  
=High Pressure Liquid Chromatography Setup=
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=High Pressure Liquid Chromatography Setup (HPLC)=
  
 
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=Results=
 
=Results=
 
Caffeine should be detected as sharp peaks at 275nm as can be observed in the image below.
 
Caffeine should be detected as sharp peaks at 275nm as can be observed in the image below.
 
[[File:Std.PNG|thumb|250px|frame|left|Caffeine Standard Spectra]]
 
[[File:Std.PNG|thumb|250px|frame|left|Caffeine Standard Spectra]]

Revision as of 15:25, 19 April 2016

HPLC method for caffeine analysis

High Pressure Liquid Chromatography Setup (HPLC)

HPLC Pump Gradient Flow Settings
HPLC Pump Solvent Settings
HPLC UV Detector General Settings
HPLC UV Detector Channel 1 Settings
HPLC UV Detector Channel 2 Settings

Results

Caffeine should be detected as sharp peaks at 275nm as can be observed in the image below.

Caffeine Standard Spectra