Difference between revisions of "Caffeine HPLC Method"
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HPLC method for caffeine analysis | HPLC method for caffeine analysis | ||
+ | =High Pressure Liquid Chromatography Setup= | ||
{|align="center" | {|align="center" | ||
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||[[File:4.PNG|thumb|250px|frame|center|HPLC UV Detector Channel 1 Settings]] | ||[[File:4.PNG|thumb|250px|frame|center|HPLC UV Detector Channel 1 Settings]] | ||
||[[File:5.PNG|thumb|250px|frame|center|HPLC UV Detector Channel 2 Settings]] | ||[[File:5.PNG|thumb|250px|frame|center|HPLC UV Detector Channel 2 Settings]] | ||
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|} | |} | ||
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+ | =Results= | ||
+ | Caffeine should be detected as sharp peaks at 275nm as can be observed in the image below. | ||
+ | [[File:Std.PNG|thumb|250px|frame|center|Caffeine Standard Spectra]] |