Difference between revisions of "Caffeine HPLC Method"

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HPLC method for caffeine analysis
 
HPLC method for caffeine analysis
  
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=High Pressure Liquid Chromatography Setup=
  
 
{|align="center"
 
{|align="center"
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||[[File:4.PNG|thumb|250px|frame|center|HPLC UV Detector Channel 1 Settings]]
 
||[[File:4.PNG|thumb|250px|frame|center|HPLC UV Detector Channel 1 Settings]]
 
||[[File:5.PNG|thumb|250px|frame|center|HPLC UV Detector Channel 2 Settings]]
 
||[[File:5.PNG|thumb|250px|frame|center|HPLC UV Detector Channel 2 Settings]]
||[[File:Std.PNG|thumb|250px|frame|center|Caffeine Standard Spectra]]
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||
 
|}
 
|}
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=Results=
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Caffeine should be detected as sharp peaks at 275nm as can be observed in the image below.
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[[File:Std.PNG|thumb|250px|frame|center|Caffeine Standard Spectra]]

Revision as of 15:25, 19 April 2016

HPLC method for caffeine analysis

High Pressure Liquid Chromatography Setup

HPLC Pump Gradient Flow Settings
HPLC Pump Solvent Settings
HPLC UV Detector General Settings
HPLC UV Detector Channel 1 Settings
HPLC UV Detector Channel 2 Settings


Results

Caffeine should be detected as sharp peaks at 275nm as can be observed in the image below.

Caffeine Standard Spectra